Journal: Horticulture Research

Article Title: Molecular dynamics of pre-germinative metabolism in primed eggplant ( Solanum melongena L.) seeds

doi: 10.1038/s41438-020-0310-8

Figure Lengend Snippet: a Experimental design showing the selected timepoints for molecular analyses. Seeds were collected during priming (HP72 protocol) at 24, 48, and 72 h, as well as at the end of dry-back (DB, 2 h). Germination of unprimed (UP) and primed (HP) seeds was monitored by collecting samples throughout imbibition at 2, 4, 8, 16, 24, 48, and 72 h. The last timepoint, corresponding to the phenological stage of radicle protrusion (RD), was anticipated at 4 days for the primed seeds, compared to unprimed (8 days). ROS levels were measured during hydropriming b and subsequent germination c at the selected timepoints using the DCFH-DA fluorescent dye. Values are expressed as mean ± SD of three independent replications with 20 seeds for each replication. Asterisks indicate statistically significant differences determined using Student’s t -test ( P < 0.05). DS dry seed, R.F.U. relative fluorescence unit, ROS reactive oxygen species, DCFH-DA dye 2′,7′-dichlorofluorescein diacetate

Article Snippet: Samples (three seeds per timepoint) were incubated for 15 min with 50 μl of 10 μM DCFH-DA and subsequently fluorescence at 517 nm was determined using a Rotor-Gene 6000 PCR apparatus (Corbett Robotics, Brisbane, Australia), setting the program for one cycle of 30 s at 25 °C.

Techniques: Fluorescence

Journal: Horticulture Research

Article Title: Molecular dynamics of pre-germinative metabolism in primed eggplant ( Solanum melongena L.) seeds

doi: 10.1038/s41438-020-0310-8

Figure Lengend Snippet: a The experimental design is shown: for each lot, the unprimed samples included dry seeds (UP) as well as rehydrated seeds collected at 2 h of imbibition (UP2), whereas the primed samples included seeds that underwent the HP72 treatment followed by dry-back (HPDB) and seeds collected at 2 h of imbibition (HPDB2). b ROS levels were measured at the selected timepoints using the DCFH-DA fluorescent dye. Values are expressed as mean ± SD of three independent replications with 20 seeds for each replication. Asterisks indicate statistically significant differences determined using Student’s t -test ( P < 0.05). DS, dry seed. R.F.U. relative fluorescence unit, ROS reactive oxygen species, DCFH-DA dye 2′,7′-dichlorofluorescein diacetate, SL seed lot

Article Snippet: Samples (three seeds per timepoint) were incubated for 15 min with 50 μl of 10 μM DCFH-DA and subsequently fluorescence at 517 nm was determined using a Rotor-Gene 6000 PCR apparatus (Corbett Robotics, Brisbane, Australia), setting the program for one cycle of 30 s at 25 °C.

Techniques: Fluorescence

Journal: Frontiers in Plant Science

Article Title: Artificial intelligence-driven systems engineering for next-generation plant-derived biopharmaceuticals

doi: 10.3389/fpls.2023.1252166

Figure Lengend Snippet: Automation and AI Tools in plant monitoring.

Article Snippet: pheno Seeder , KR 10 scara R600-Z300 robot (KUKA Roboter GmbH, Germany) , Oscar F-810C Camera (Allied-Vision Technologies, GmbH, Germany) , Seed projected area, length, width and color , B. napus (rapeseed), H. vulgare (barley) and A. thaliana , .

Techniques: Imaging, High Throughput Screening Assay, Software, Mutagenesis